ABSTRACT The objective of this study was to investigate the acidic soil physical and chemical alteration associated to vinasse application under laboratory conditions. The experiment was distributed in a factorial scheme (5 × 3), in a completely randomized design, with four replicates. The beneficial effects of five vinasse doses (without vinasse [0, only water], 50, 100, 150, and 200 m3/ha) in enhancing the physical and chemical properties of three acidic soils: Oxisol (SO), Ultisol (SU), and Argisol (SA) were studied after 30 days of incubation. Soil samples were collected in the 0-20 cm depth layer to determine physical and chemical properties such as pH (CaCl2), total acidity (AT), exchangeable acidity (Al3+), base cations (K+, Ca2+, and Mg2+), sums of bases (SB), cations exchange capacity (CIC), base saturation index (V%), and saturation of potassium (K), calcium (Ca), magnesium (Mg), and aluminum (Al). Results indicated that vinasse application enhanced the physical and chemical properties of the studied soils, since pH, base cations, SB, CIC, V%, and saturation of K, Ca, and Mg were increased, while AT, Al3+, and Al saturation were decreased. The most important effects in the improvement of physical and chemical properties of acidic soils were observed with a dose of 200 m3/ha of vinasse. The findings of this study suggest that vinasse could be a low-cost effective strategy as an alternative source for balancing soil acidity and would be an appropriate option for minimizing environmental pollution.

RESUMEN El objetivo de este estudio fue investigar las alteraciones físicas y químicas de los suelos ácidos asociadas a la aplicación de vinaza en condiciones de laboratorio. Los efectos beneficiosos de cinco dosis de vinaza (sin vinaza [0, solamente agua], 50, 100, 150 y 200 m3/ha), fueron estudiadas en el mejoramiento de las propiedades físicas y químicas de tres suelos ácidos: Oxisol (SO), Ultisol (SU) y Argisol (SA), después de 30 días de incubación. Las muestras de suelos fueron recolectadas en la capa de 0-20 cm de profundidad para la determinación de propiedades físicas y químicas como pH (CaCl2), acidez total (AT), acidez intercambiable (Al3+), cationes bases (K+, Ca2+ y Mg2+), suma de bases (SB), capacidad de intercambio catiónico (CIC), índice de saturación de las bases (V%), y saturación de potasio (K), calcio (Ca), magnesio (Mg) y aluminio (Al). Los resultados indicaron que la aplicación de vinaza mejoró las propiedades físicas y químicas de los suelos estudiados, porque se incrementaron tanto el pH como los cationes bases, la SB, la CIC, el V% y la saturación de K, Ca y Mg, y disminuyeron la AT, Al3+ y saturación de Al. Además, los efectos más importantes en el mejoramiento de las propiedades físicas y químicas de los suelos ácidos se observaron en la dosis de 200 m3/ha de vinaza. Los hallazgos de este estudio sugieren que la vinaza podría ser una estrategia efectiva de bajo costo como fuente alternativa para balancear la acidez del suelo y constituiría una opción apropiada para disminuir la contaminación ambiental.

ABSTRACT Soil nutrient availability and interpretations of nutrient contents are based on the results obtained from specific extraction methods used in routine testing laboratories. The development of new extraction methods and new extractants with better accuracy is particularly important to determine the correct fertilizer rates to be applied. For this purpose, this study aims to synthesize, characterize and evaluate the predictive capacity of cellulose acetate film containing calcinated layered double hydroxide (CAF-LDH-c), as a new extractant and a new method of P extraction in soils. Different analyses techniques were used to characterize the CAF-LDH-c sample, including X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and scanning electronic microscopy. Soils were collected from twelve areas with different management and initial availability of P. The soils were subjected to six rates of P and were cultivated with plants. The predictive capacity of CAF-LDH-c, as a new extractant of P in different soils, was evaluated and compared to Mehlich-1 (M-1), Mehlich-3 (M-3), and Mixed Exchange Resin (MER) extractants. Chemical analyses performed on CAF-LDH-c showed that LDH in powder form was incorporated into the cellulose acetate film. There was a linear relation between P uptake by plants and extractable soil P in the soil by CAF-LDH-c, M-1, M-3, and MER extractants. The significant correlations between extracted P in the soil and P uptake by plants for CAF-LDH-c showed the efficacy of the new extractor and the newly proposed method for different types of soils. The results from the present study confirm the possibility of using CAF-LDH-c extractant as a new methodology to evaluate the availability of P in the soil for plant cultivation.

ABSTRACT Cedro-rosa (Cedrela fissilis) is native to Brazil with great potential for seedling production. However, soil correction is necessary to improve its performance, due to the edaphoclimatic conditions in Brazil, where soils have a high weathering degree and large amounts of Fe and Al oxides. Here, we evaluated the combined effect of soil acidity correction by liming and phosphate fertilization using single superphosphate on the growth and development of cedro-rosa seedlings cultivated in Amazonian soil. The study was carried out in a greenhouse at the Universidade Federal Rural da Amazônia (UFRA) – Campus Capitão Poço, between July and December 2019. Different levels of base saturation (V%) showed no influence on the variables analyzed. On the other hand, phosphorus (P), calcium (Ca), and sulfur (S) supplied by single superphosphate significantly influenced growth and development of cedro-rosa seedlings. The optimum P levels for stem diameter (SD), leaves number (LN), root dry matter (RDM), stem dry matter (StDM), leaf dry matter (LDM), total dry matter (TDM) were 405, 286, 385, 421.87, 393.75, 445 mg dm-3 of P, respectively. Seedling quality (DQI) showed a positive linear response to levels of P, Ca, and S in single superphosphate. Thus, fertilization with P was necessary at the nursery stage for the cultivation of the species in soil of low chemical fertility. For better quality of cedro-rosa seedlings grown in pots, the application of 400 mg dm-3 of P is recommended.

ABSTRACT Studies aimed at mitigating environmental impacts have received attention in recent years. The use of biochar obtained from the pyrolysis is an alternative for the recovery of organic materials that usually are directly discarded in the environment. Biochar has the potential to recover nutrients from wastewater, and in the latter case, the reuse or recycling of biochar as a controlled-release fertilizer could be an option. However, the adsorption characteristics of the ions into different biochars have not been known. The aim of this study was to evaluate the ions adsorption in biochar derived from discarded potatoes, as well as to make physical and chemical characterization of the produced materials. The biochar derived from discarded potatoes was produced by pyrolysis at 450 °C under an N2 atmosphere. In order to define its ionic profile, the adsorption of HnPO4(3–n)–, Ca2+, Mg2+, and Al3+ was tested in pure biochar, as well as adsorption of HnPO4(3–n)–in biochar after it was doped with Al3+. The desorption of these ions was tested by washing with deionized water, and the KCl (1.0 mol·L–1) and Mehlich-1 extractors. In absolute values, the adsorption order of the ions was Al3+ (in acid pH value) = Ca2+ > Al3+ > HnPO4(3–n)–(biochar doped with Al3+) > Mg2+ > HnPO4(3–n)– (biochar without doping). The adsorption of HnPO4(3–n)– was positively influenced by the doping of biochar with Al3+. The biochar has preferential characteristics for cations adsorption, and the doping process can aid in adsorption of anions.

Resumo A adesão ao tratamento medicamentoso é fundamental para o sucesso da terapia. Objetivou-se desenvolver um instrumento de avaliação da Adesão Medicamentosa no Diabetes Melitus (AMDM) e avaliar suas propriedades psicométricas. Trata-se de um estudo metodológico de desenvolvimento e avaliação do AMDM, instrumento de medida de um evento relacionado à saúde. A versão final do AMDM constou de 20 questões que apresenta duas partes, a primeira denominada AMDM-I, com 12 questões e a segunda parte, denominada AMDM-II, com 8 questões. Realizou-se validação do conteúdo e as estimativas de confiabilidade (Alfa de Cronbach/Kappa ponderado). O AMDM apresentou boa validade de conteúdo, teste alfa de Cronbach=0,71, o Kappa ponderado variou entre 0,76 e 1,0, e a validade preditiva obteve sensibilidade=73,2% e especificidade=63,3%. O AMDM foi considerado de aplicação fácil e dinâmica, adequado ao contexto em que foi aplicado. No AMDM foram considerados itens relacionados ao comportamento dos participantes em relação ao controle do seu estoque de medicamentos, fidelidade à prescrição médica, e uso de bebidas alcoólicas; e às dificuldades na aquisição de medicamentos, inclusive por meio do Sistema Único de Saude (SUS), sistema de saúde vigente no Brasil. Outros instrumentos existentes não abordaram tais questões. Concluiu-se que o AMDM apresentou confiabilidade e validade aceitáveis, além de boa interpretabilidade, mostrando-se capaz de avaliar o que se propõe.

Abstract Adherence to medication treatment is fundamental to the success of therapy. The objective was to develop an instrument for the evaluation of Medicinal Adherence in Diabetes Mellitus (AMDM) and to evaluate its psychometric properties. This is a methodological study of the development and evaluation of AMDM, an instrument for measuring a health-related event. The final version of AMDM consisted of 20 questions with two parts, the first called AMDM-I, with 12 questions and the second part, called AMDM-II, with 8 questions. Content validation and reliability estimates (Cronbach's alpha / weighted Kappa) were performed. The AMDM showed good content validity, Cronbach's alpha test = 0.71, the weighted Kappa ranged from 0.76 to 1.0, and the predictive validity obtained sensitivity = 73.2% and specificity = 63.3%. AMDM was considered to be easy and dynamic to apply, appropriate to the context in which it was applied. At AMDM, items related to the participants' behavior in relation to the control of their stock of medicines, compliance with medical prescription, and use of alcoholic beverages were considered; and difficulties in the acquisition of medicines, including through the Unified Health System (SUS), a health system in force in Brazil. Other existing instruments have not addressed these issues. It was concluded that the AMDM presented acceptable reliability and validity, in addition to good interpretability, showing itself capable of evaluating what is proposed.

RESUMO Este artigo tem por objetivo compreender como as crianças em idade pré-escolar constroem as suas relações étnico-raciais em uma creche universitária na cidade do Rio de Janeiro. Considera-se neste estudo três dimensões que emergiram da pesquisa: as microações afirmativas expressivas registram a autoexpressão das crianças; as microações afirmativas inclusivas consideram a participação das crianças negras e não negras em situação de inclusão e as microações afirmativas formativas tratam do desenvolvimento profissional docente. A pesquisa aponta para a importância das microações afirmativas no desenvolvimento de práticas sociais antirracistas e pretende contribuir para os estudos da infância e para educação das relações étnico-raciais.

ABSTRACT This article aims to understand how pre-school children build their ethnic-racial relationships in a university day care center in the city of Rio de Janeiro. This study considers three dimensions that emerged from the research: the expressive affirmative micro actions, register the children’s self-expression; the inclusive affirmative micro actions consider the participation of black and non-black children in the situation of inclusion and formative affirmative micro actions, it is the professional development of teachers. Research points to the importance of affirmative micro actions in the development of anti-racist social practices and aims to contribute to childhood studies and the education of ethnic-racial relations.

ABSTRACT An experiment with 23 diets was performed to evaluate the effect of digestible lysine (Lys), digestible methionine + cysteine (Met+Cys), and digestible threonine (Thr) on egg production of H&N Brown second-cycle laying hens (SCLH) for 20 weeks (92-111 weeks of age) in cages under environmental conditions. Body weight (BW), feed intake (FI), feed conversion ratio (FCR), egg weight (EW), number of hen-housed eggs, and livability were also evaluated during the experiment. Diets were formulated from a central composite design that combined five levels of Lys, Met+Cys, and Thr ranging from 727 to 1159, 662 to 1055, and 552 to 882 mg/kg, respectively. Egg production (EP) data were evaluated through three different modeling strategies: egg production models, multivariate polynomial models, and artificial neural networks (ANN). A cascade-forward neural network with log-sigmoid transfer function was selected as the best model according to goodness-of-fit statistics in both identification and validation data. One of the best scenarios for EP of H&N Brown SCLH under specific outdoor conditions was established at Lys, Met+Cys, and Thr levels of 1138, 1031, and 717 mg/hen·day, respectively. The ANN model may be an appropriate tool to study and predict EP of H&N Brown SCLH based on the combination of three different levels of essential digestible amino acids. The strategies included in this work may contribute to improving poultry performance based on modeling techniques to study other production parameters in terms of different nutritional requirements and productive conditions.

RESUMO Embora os efeitos do alumínio sobre o teor e acúmulo de nutrientes minerais nas culturas sejam tenham sido estudados, mas nada se sabe sobre seu efeito na eficiência nutricional da cana-de-açúcar. Diante disso, o objetivo deste trabalho foi avaliar o efeito da toxidez de alumínio nas eficiências nutricionais, teor e acúmulo de nutrientes e crescimento de mudas de cana-de-açúcar. Para o ensaio foram utilizadas mudas de cana do genótipo IACSP95-5000 as quais foram obtidas a partir de minitoletes (3 cm) contendo uma gema cada um. As mudas foram submetidas aos tratamentos em delineamento inteiramente casualizado com 8 concentrações de Al+3 (0, 10, 20, 30, 40, 50, 60, 70 mg L-1) em solução nutritiva com 3 repetições, perfazendo 24 unidades experimentais. As análises determinaram o conteúdo, acúmulo e absorção, transporte e eficiência de uso de macronutrientes, micronutrientes e alumínio, além da produção de matéria seca. Os resultados mostram que o Al afetou todas as variáveis analisadas, com diminuição na eficiência nutricional dos macronutrientes e micronutrientes e diminuição drástica no acúmulo de macronutrientes na parte aérea, o que refletiu na diminuição da matéria seca das plantas. O sistema radicular foi o mais afetado, com uma diminuição no crescimento de até 60%. O alumínio reduziu a eficiência de uso de nutrientes em ordem decrescente: Ca (69%)> N (60%)> K (59%)> Mg (50%)> S (49%)> P (40%). Quanto aos micronutrientes, observou-se a seguinte ordem decrescente: Fe (73%)> Zn (59%) = Cu (59%)> Mn (25%).

ABSTRACT Although the effects of aluminum on the content and accumulation of mineral nutrients in crops have been studied, but nothing is known about its effect on the nutritional efficiency of sugarcane. Therefore, this study aimed to evaluate the effect of aluminum toxicity on nutritional efficiency, nutrient accumulation, and growth of sugarcane seedling. Sugarcane seedlings of the genotype IACSP95-5000, obtained from one-bud mini-cuttings (3 cm), were used in the test. Seedlings were subjected to treatments in a completely randomized design, with eight Al concentrations in the nutrient solution (0, 10, 20, 30, 40, 50, 60, and 70 mg L−1) and three replicates, totaling 24 experimental units. Analyses determined the contents, accumulation, and absorption, transport, and use efficiency of macronutrients, micronutrients, and aluminum, in addition to dry matter production. The results show that Al affected all variables analyzed, with a decrease in the nutritional efficiency of macronutrients and micronutrients and a drastic decrease in the accumulation of macronutrients in the aerial part, which reflected in the decrease in the dry matter of the plants. The root system was the most affected, with a decrease in growth of up to 60%. Aluminum reduced the use efficiency of nutrient in decreasing order: Ca (69%)> N (60%)> K (59%)> Mg (50%)> S (49%)> P (40%). As for micronutrients, the following decreasing order was observed: Fe (73%)> Zn (59%) = Cu (59%)> Mn (25%).

Abstract Information Technology (IT) governance in small and medium-sized businesses is a subject that still requires more effort. Many of the studies that deal with this subject refer to large companies. IT governance structures, originally developed for the context of large enterprises such as COBIT, ISO/IEC 35800 or ITIL, when applied to the context of small and medium enterprises, lead to undesirable results and failures in the deployment process. The objective of this work was to perform a systematic mapping on IT governance in small and medium enterprises between 2007 and 2017. Systematic mapping studies are useful for categorizing and summarizing the existing information concerning a research question in an unbiased manner. Thus, from an initial set of 63 papers, a total of 17 research papers were selected for the mapping study. The results obtained allowed us to reach conclusions concerning the state-of-the-art of IT governance mechanisms, theories applied in the context and the consequences of IT governance. From the results of this study it was possible to perceive the low use of relational mechanisms of IT governance in the studied context, the constant need to adapt frameworks such as COBIT and ITIL, so that they can adapt to the reality of SMEs, the wide variety of theoretical approaches and the importance, for SMEs, of the concept of IT value for the business.

Resumen Este estudio tuvo como objetivo evaluar el confort térmico para trabajadores, en tres edificaciones de procesamiento húmedo de café en Colombia, utilizando el índice WBGT, y análisis de temperatura y humedad relativa a través de mapeo. Los límites recomendados del WBGT fueron excedidos en la actividad de abastecimiento de combustible de la secadora en la Edificación 3. Las tres edificaciones presentaron variabilidad higrotérmica espacial debida a la secadora de café, y a problemas con la ventilación, volumen de control y resistencia térmica de materiales en los edificios 2 y 3. Para favorecer la transferencia de calor de la secadora al ambiente exterior, y hacer más homogéneo el ambiente térmico de las edificaciones, se recomienda instalar puentes térmicos alrededor de esta zona, y para hacer más eficiente la evacuación de vapor y calor, aumentar el área de ventilación natural en las edificaciones 2 y 3, e instalar claraboyas de ventilación sobre la secadora.

Abstract This study aimed to assess thermal comfort for workers in three buildings for wet coffee processing in Colombia, using the WBGT index, and temperature and relative humidity analysis through mapping. The limits recommended by the WBGT index were exceeded during the activity of putting fuel in the dryer in Building 3. The three buildings presented spatial hygrothermal variability due to the coffee dryer, as well as problems with ventilation, volume control, and the thermal resistance of materials in Buildings 2 and 3. In order to favor heat transfer from the dryer to the outside environment, and to make the thermal environment of these buildings more homogeneous, it is recommended to install thermal bridges around this area, to make more efficient the evacuation of steam and heat, as well as to increase the natural ventilation area in Buildings 2 and 3, and install ventilation skylights over the dryer.

Resumo Este estudo tem como objetivo avaliar o conforto térmico para trabalhadores em três instalações de processamento úmido de café na Colômbia, utilizando o índice WBGT, a análise de temperatura e a umidade relativa através de mapeamento. Os limites recomendados do WBGT foram excedidos na atividade de abastecimento de combustível da secadora na Instalação 3. As três edificações apresentaram variabilidade higrotérmica espacial devidas à secadora de café e a problemas com ventilação, volume de controle e resistência térmica de materiais nos edifícios 2 e 3. Para favorecer a transferência de calor da secadora para o ambiente exterior e tornar mais homogêneo o ambiente térmico das edificações, recomenda-se instalar pontes térmicas ao redor dessa zona e, para tornar mais eficiente a evacuação de vapor e calor, aumentar a área de ventilação natural nas edificações 2 e 3, bem como instalar claraboias de ventilação sobre a secadora.

ABSTRACT Chemical investigation of the aqueous fraction of the ethanol extract from the Brazilian endemic marine sponge Clathria (Clathria) nicoleae Vieira de Barros, Santos & Pinheiro, 2013, Microcionidae, sampled from a 55 m deep rhodolith bed at the Amazon River mouth, led to the isolation of a new hexapeptide, clathriamide (1). HP-20 resin was used to capture compound 1 from the aqueous fraction, which was purified by additional chromatographic steps. The absolute configuration of the amino acids of 1 was determined by advanced Marfey's analysis using 5-fluoro-2,4-dinitrophenyl-Nα-L-tryptophanamide. The amino acid derivatives analyzed by ultra-performance liquid chromatography coupled to a mass spectrometry using a C8 column enabled a good chromatographic resolution of L-Ile and L-allo-Ile, previously unfeasible using C18 column.

Resumo Introdução Adultos usam serviços odontológicos públicos ou privados. Objetivo Identificar fatores associados ao uso dos serviços odontológicos provenientes do SUS entre adultos brasileiros, considerando aspectos contextuais e individuais. Método Foram utilizados dados contextuais de 177 municípios do DATA/SUS, do IBGE, do Atlas Brasil e do Projeto SB Brasil/2010, bem como dados individuais de uma amostra pobabilistica do SB Brasil/2010 coletados por dentistas calibrados. O SPSS 19.0® foi utilizado nas análises descritivas e bivariadas, e o STATA 14.0®, na análise multinível. Resultados A prevalência do uso de serviços no SUS foi de 37,9%. O uso de serviços no SUS foi maior nos municípios com pior acesso à coleta de lixo, em indivíduos com menor escolaridade e menor renda familiar, em negros/pardos/amarelos/indígenas, naqueles com dentes cariados e nos que avaliaram a consulta odontológica como regular/ruim/péssima. Já o uso foi menor nos municípios com menor cobertura de equipes de saúde bucal, entre os mais velhos e em indivíduos do sexo masculino. O maior uso entre os mais vulneráveis sugere equidade. Conclusão Constatou-se efeitos contextuais e individuais sobre o comportamento dos adultos quanto ao tipo de serviço odontológico utilizado que devem ser considerados nas políticas de saúde bucal.

Abstract Background Adults who demand for public or private dental services. Objective Factors associated with the use of dental services from SUS were identified among Brazilian adults, considering context and individual factors. Method It was used contextual data from 177 municipalities of DATA/SUS, IBGE, Atlas Brazil and SB Brazil / 2010 Project; and individual data from SB Brazil/2010. SPSS 19.0® was used in descriptive and bivariate analyzes and STATA 14.0® in multilevel analysis. Results The prevalence of use of services in SUS was 37.9%. The use of services in SUS was higher in municipalities with poor access to waste collection and lower in those with lower coverage of oral health teams. The use was lower among older men. Among those with lower schooling, lower family income, among blacks/brown/yellows and Indigenous, those with decayed teeth, and those who evaluated the dental appointment as regular/bad or bad. The greater use among the most vulnerable suggests equity. Conclusion Contextual and individual effects on the behavior of adults regarding the type of dental service used, which should be considered in oral health policies were verified in this study.

RESUMO Estudos demonstram que a utilização do cloro em estações de tratamento de água (ETA) pode contribuir para a formação de subprodutos orgânicos halogenados indesejados, tais como os trialometanos (TAM), quando há presença de matéria orgânica algogênica, composta de algas e cianobactérias. A Microcystis aeruginosa é uma espécie de cianobactéria com frequentes registros em eventos de florações no país e é relacionada com a formação de TAM durante a cloração da água. Desse modo, este estudo teve como objetivo o desenvolvimento e a validação do método analítico por extração líquido-líquido para detecção e quantificação de TAM por cromatografia gasosa acoplada à espectrometria de massas (ELL-CG-EM), bem como a aplicação deste para avaliar a formação de TAM em ensaios de cloração de células de Microcystis aeruginosa, simulando situações em ETA. O método obteve baixo tempo de análise (< 12 minutos), excelente seletividade, precisão, repetitividade e sensibilidade, com possibilidade de aplicação para análises de rotina em detrimento de outras técnicas consideradas mais automatizadas. Foram observadas alta demanda de cloro durante os ensaios e elevada concentração dos subprodutos quando submetida à dose de cloro gasoso (Cl2) de 2,5 e 5 mg.L-1, com destaque para o triclorometano, sendo outras espécies de monitoramento obrigatório não detectadas ou não formadas, o que pode ser justificado pela ausência de bromo.

ABSTRACT Studies have shown that the use of chlorine in water treatment plants (WTPs) can contribute to formation of undesirable halogenated organic by-products, such as trihalomethanes (THMs), when algae and cyanobacteria are present. Microcystis aeruginosa is a cyanobacteria specie with frequent recordings of flowering events in the country and is related to the formation of THMs during water chlorination. Thus, the objective of this study was to develop and validate Liquid-Liquid Extraction the analytical method for detection and quantification of THMs by gas chromatography coupled to mass spectrometry (LLE-GC-MS), as well as the application of the method to evaluate the formation of TAMs from Microcystis aeruginosa cells chlorination tests, simulating situations in WTPs. The method obtained low time of analysis (< 12 minutes), excellent selectivity, precision, repeatability and sensitivity, with possibility of application for routine analysis to the detriment of other techniques considered more automated. High chlorine demand was observed during the tests and high concentration of by-products when submitted to the chlorine gas (Cl2) dose of 2.5 and 5 mg.L-1, with emphasis on trichloromethane. Other species of mandatory monitoring were not detected or not formed, justified by the absence of bromine.

Abstract Purpose: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. Methods: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology. Results: The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group. Conclusions: This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.

Resumo A reabilitação protética foi prevista pela política nacional de saúde bucal em função da alta prevalência de perda de todos os dentes e do baixo uso de prótese dentária entre idosos. Há necessidade de se avaliar o impacto dessa política de reabilitação. Portanto, objetivou-se investigar se o tipo de serviço odontológico utilizado foi um dos fatores associados ao uso de prótese dentária total entre idosos. Trata-se de um estudo transversal conduzido em uma amostra probabilística de idosos (60 anos ou mais) residentes na zona urbana de um município brasileiro de pequeno porte populacional por examinadores calibrados. Realizaram-se análises descritiva, bivariada e múltipla (Regressão logística - odds ratio - OR). Participaram 287 idosos, destes, 186 (64,8%) faziam uso de prótese dentária total. O uso de prótese dentária total foi associado a maiores chances de uso de serviços odontológicos supletivos ou particulares (OR = 4,19; p < 0,001) e a presença de lesão no palato (OR = 7,52; p = 0,002) e, também, a menores chances em idades maiores ou iguais a 73 anos (OR = 0,52; p = 0,023) e entre os com comprometimento na dimensão incapacidade física do OHIP-14 (OR = 0,20; p = 0,002). O uso de prótese dentária total foi associado ao tipo de serviço odontológico entre idosos, sendo maior o uso entre usuários dos serviços supletivos ou particulares.

Abstract Oral rehabilitation with dental prosthesis was mandated by national oral health policy in Brazil, due to the high prevalence of total teeth loss and low use of dental prosthesis among the elderly. There is a pressing need to assess the impact of this rehabilitation policy. An attempt was therefore made to investigate if the dental service used was one of the factors associated with the use of total dental prosthesis among the elderly. To achieve this, a cross-sectional study using a probabilistic sample of elderly (60 years and above) urban-area residents of a small city was conducted by qualified examiners. Descriptive, bivariate and multiple (logistic regression/odds ratio - OR) analyses were conducted on 287 elderly people, of which 186 (64.8%) used dental prosthesis. The use of total dental prosthesis was associated with a higher probability of being attended by private dental services (OR = 4.19; p < 0.001) and the presence of lesions on the palate (OR = 7.52; p = 0.002). Conversely, it was associated with lower probability in ages greater than or equal to 73 years (OR = 0.52; p = 0.023) and among those with impairment of OHIP-14 (OR = 0.20; p = 0.002) in the physical disability dimension. The use of total dental prosthesis was associated with the type of dental service used by the elderly, it being greater among private service users.